Nucleic Acid Mass Conc. ⇄ Molarity

What Is the Nucleic Acid Concentration Converter?

The Nucleic Acid Concentration Converter is a quantitative tool designed to convert nucleic acid length into molar concentration and mass concentration. By combining nucleic acid length with solution volume or amount, the tool enables accurate preparation and analysis of DNA and RNA solutions commonly used in molecular biology experiments.

Core Functionality

Length-Based Conversion:
Convert nucleic acid length directly into molar concentration (mol/L) or mass concentration (g/L).
Bidirectional Calculations:
Supports conversion between nucleic acid length, molar concentration, and mass concentration.

Supported Nucleic Acid Types

Double-stranded DNA (dsDNA)
Single-stranded DNA (ssDNA)
Single-stranded RNA (ssRNA)

Molecular Weight Calculation Methods

The molecular weight of nucleic acids can be calculated using one of three commonly accepted methods. Users may freely select the method that best matches their experimental standards.

Method 1 (Average Base Weight):
dsDNA MW = Length (bp) × 660 g/mol
ssDNA MW = Length (nt) × 330 g/mol
ssRNA MW = Length (nt) × 340 g/mol

Method 2 (Empirical Formula):
dsDNA MW = Length × 615.96 g/mol + 36.04 g/mol
ssDNA MW = Length × 307.97 g/mol + 18.02 g/mol
ssRNA MW = Length × 320.47 g/mol + 18.02 g/mol

Method 3 (Alternative Empirical Formula):
dsDNA MW = Length × 617.96 g/mol + 36.04 g/mol
ssDNA MW = Length × 308.97 g/mol + 18.02 g/mol
ssRNA MW = Length × 321.47 g/mol + 18.02 g/mol

How It Works

Input Nucleic Acid Length:
Enter base pairs (bp) for dsDNA or nucleotides (nt) for ssDNA and ssRNA.
Select Nucleic Acid Type:
Choose between dsDNA, ssDNA, or ssRNA.
Select Molecular Weight Method:
Choose one of the three molecular weight calculation formulas.
Calculate Concentrations:
The tool automatically converts length into molecular weight and calculates molar concentration and mass concentration using standard chemical equations.

Experimental Significance

Accurate conversion between nucleic acid length, molar concentration, and mass concentration is fundamental for molecular cloning, qPCR, sequencing library preparation, and transfection experiments. This tool standardizes calculations, reduces manual errors, and improves reproducibility across experiments and laboratories.