OD₂₆₀ Nucleic Acid Concentration

Convert OD₂₆₀ to nucleic acid Concentration

OD260 Nucleic Acid Concentration Calculator

This is a simple tool to calculate nucleic acid concentration based on OD260 absorbance.

How It Works

Enter the OD260 value of your nucleic acid solution and select the nucleic acid type. The tool will convert absorbance to mass concentration using the standard coefficients below.

Conversion Formula

OD260 absorbance to nucleic acid concentration:
1 OD260 Unit = 50 µg/mL for dsDNA
1 OD260 Unit = 40 µg/mL for ssRNA
1 OD260 Unit = 35 µg/mL for ssDNA
1 OD260 Unit = 20 µg/mL for ssOligo

Applications

This tool is useful for quick estimation of nucleic acid concentrations in molecular biology experiments, such as preparing stock solutions, performing qPCR, or designing in vitro transcription reactions.

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