DNA Copy Number Dilution
This tool calculates DNA copy number based on nucleic acid length and stock concentration (ng/μL).
By entering DNA length, stock concentration, target final concentration (copies/μL), and final volume, it determines the required stock volume.
It automatically converts mass concentration to copy number and provides a precise dilution scheme for preparing the target solution.
What is DNA Copy Number & Concentration Calculator?
The DNA Copy Number & Concentration Calculator is a versatile nucleic acid quantification tool that allows precise conversions between nucleic acid length, mass concentration, copy number concentration, and molarity, while also generating accurate dilution schemes based on target concentrations.
This tool is not limited to dilution calculations—it can also be used for nucleic acid quantification, standard preparation, and experimental design, making it a universal assistant for molecular biology workflows.
Core Functionalities
- Molecular Weight and Copy Number Calculation:
Enter nucleic acid length and the tool automatically calculates molecular weight and the number of copies per nanogram (ng). - Copy Number and Molarity Calculation:
Enter nucleic acid concentration (ng/μL) to calculate copies per microliter (copies/μL) and the corresponding molarity (mol/L) of the solution. - Dilution Scheme for Target Concentrations:
Enter target copy number concentration or molarity, and the calculator computes the required stock volume and provides a clear dilution plan. - Multi-purpose Design:
This tool supports both dilution planning and nucleic acid quantification, and is not restricted to a single application.
Supported Nucleic Acid Types
- Double-stranded DNA (dsDNA)
- Single-stranded DNA (ssDNA)
- Single-stranded RNA (ssRNA)
Molecular Weight Calculation Methods
To accommodate different experimental preferences and accuracy requirements, three commonly used nucleic acid molecular weight calculation models are provided, which can be freely selected:
dsDNA MW = Length(bp) × 660 g/mol
ssDNA MW = Length(nt) × 330 g/mol
ssRNA MW = Length(nt) × 340 g/mol
Method 2 (Adjusted Average Formula):
dsDNA MW = Length × 615.96 g/mol + 36.04 g/mol
ssDNA MW = Length × 307.97 g/mol + 18.02 g/mol
ssRNA MW = Length × 320.47 g/mol + 18.02 g/mol
Method 3 (Alternative Adjusted Formula):
dsDNA MW = Length × 617.96 g/mol + 36.04 g/mol
ssDNA MW = Length × 308.97 g/mol + 18.02 g/mol
ssRNA MW = Length × 321.47 g/mol + 18.02 g/mol
Experimental Significance
Accurate calculation of DNA copy number and concentration is critical for qPCR standard curve construction, nucleic acid quantification, standard preparation, and reproducibility control. By automating calculations and supporting multiple models, this tool reduces human error and improves reliability in experimental design and execution.
