Nucleic Acid Moles Converter

Calculate nucleic acid mass or molar amount based on DNA/RNA length.

Enter the nucleic acid length and either the number of moles or the mass to calculate the missing parameter.

This tool simplifies conversion between nucleic acid mass and molar quantity for molecular biology experiments.

What is the Nucleic Acid Moles Converter?

This tool allows you to calculate the mass or molar amount of nucleic acids based on DNA or RNA length. It is designed for molecular biology experiments to simplify conversions between nucleic acid mass and molar quantity.

Supported Nucleic Acid Types

  • Double-stranded DNA (dsDNA)
  • Single-stranded DNA (ssDNA)
  • Single-stranded RNA (ssRNA)

Molecular Weight Calculation Methods

You can choose from three calculation methods:

Method 1:
dsDNA MW = Length(bp) × 660 g/mol
ssDNA MW = Length(nt) × 330 g/mol
ssRNA MW = Length(nt) × 340 g/mol
Method 2:
dsDNA MW = Length × 615.96 g/mol + 36.04 g/mol
ssDNA MW = Length × 307.97 g/mol + 18.02 g/mol
ssRNA MW = Length × 320.47 g/mol + 18.02 g/mol
Method 3:
dsDNA MW = Length × 617.96 g/mol + 36.04 g/mol
ssDNA MW = Length × 308.97 g/mol + 18.02 g/mol
ssRNA MW = Length × 321.47 g/mol + 18.02 g/mol

How It Works

  • Input Nucleic Acid Length:
    Enter the length in base pairs (bp) for dsDNA or nucleotides (nt) for ssDNA/ssRNA.
  • Input Known Parameter:
    Enter either nucleic acid mass or molar amount.
  • Choose Nucleic Acid Type:
    Select dsDNA, ssDNA, or ssRNA.
  • Choose Calculation Method:
    Pick one of the three molecular weight calculation methods.
  • Instant Calculation:
    The tool automatically calculates the missing parameter (mass or moles) in real-time.

Significance in Molecular Biology

Accurate nucleic acid mass and molar calculations are essential for preparing solutions, performing quantitative PCR, cloning, and other molecular biology experiments. This converter simplifies these calculations, reducing experimental errors and improving reproducibility.

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