Preparation NP-40 Lysis Buffer
This guide describes the preparation of NP-40 Lysis Buffer at a defined molarity for laboratory use.
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NP-40 Lysis Buffer is commonly used for cell lysis and protein extraction.
NP-40 Lysis Buffer – Composition
| Name | Formula | Concentration | CAS |
|---|---|---|---|
| Tris-Cl (pH 8.0) | C₄H₁₁NO₃ | 50 mM | 77-86-1 |
| Sodium chloride | NaCl | 150 mM | 7647-14-5 |
| NP-40 | (C₂H₄O)nC₁₄H₂₂O | 1% (v/v) | 26027-38-3 |
Sterilization & Storage
- Sterilization: 0.22 μm filtration or autoclave
- Storage: Room temperature
Tips
- Various protease inhibitors and phosphatase inhibitors may be added as needed. Buffers containing inhibitors should be stored at −20°C.
- Add PMSF to a final concentration of 1 mM within a few minutes prior to use.
- Triton X-100 may also be used as a substitute for NP-40.
- If autoclaving is performed, add NP-40 after sterilization.
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