Preparation Solution III (Plasmid isolation)
This guide describes the preparation of Solution III (Plasmid isolation) at a defined molarity for laboratory use.
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Solution III is the neutralization buffer used in plasmid isolation.
Solution III (Plasmid Isolation) – Composition
| Name | Formula | Concentration | CAS |
|---|---|---|---|
| Potassium acetate | C₂H₃O₂K | 3 M | 127-08-2 |
| Acetic acid | CH₃COOH | 2 M | 64-19-7 |
Function of Solution III
Solution III neutralizes the alkaline lysis mixture from Solution II, precipitating chromosomal DNA, proteins, and cell debris while keeping plasmid DNA in solution.
Preparation Tips, Sterilization, and Storage
- By adding 3 M potassium acetate and 2 M acetic acid, the total acetate concentration of the solution is 5 M. Many references simply describe this as 5 M acetate, which can be misleading.
- Sterilization: autoclave.
- Storage: 4°C.
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