Preparation Tris-Buffered Saline (TBS)
This guide describes the preparation of Tris-Buffered Saline (TBS) at a defined molarity for laboratory use.
The following content was generated by AI and has not been strictly verified; it may contain inaccuracies. All information in the BioCalculator App has been manually curated and carefully validated. You can download the App to view it.
TBS buffer is commonly used in molecular biology experiments as a general-purpose buffer.
Tris-Buffered Saline (TBS) – Composition
| Name | Formula | Concentration | CAS |
|---|---|---|---|
| Tris base | C₄H₁₁NO₃ | 20 mM | 77-86-1 |
| Sodium chloride | NaCl | 150 mM | 7647-14-5 |
Sterilization & Storage
- Sterilization: Autoclave (optional)
- Storage: Room temperature
Tips
- White flocculent precipitates may form during low-temperature storage. Warm in a water bath until fully dissolved before use.
- At a 1X concentration, the addition of 0.05% (v/v) Tween 20 yields TBST Buffer.
- For long-term storage, keep at 4°C.
Preview
