Ammonium sulfate Stock Solution

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Stock Solution Preparation Guide – Ammonium Sulfate ((NH₄)₂SO₄) Solution

Ammonium sulfate ((NH₄)₂SO₄) is an inorganic salt widely used in biochemical and analytical laboratories. It dissolves readily in water, forming clear solutions frequently employed for salting‑out proteins, preparing ionic strength adjustment solutions, or general reagent stock solutions. Its solubility in water at 20 °C is high (≈76 g per 100 mL), and the solid appears as white crystalline powder.

Objective

Preparation of a defined concentration stock solution of ammonium sulfate (e.g., 1 M).

Composition

• Ammonium sulfate ((NH₄)₂SO₄) aqueous solution of desired molarity

Required Materials

• Ammonium sulfate ((NH₄)₂SO₄) solid
• Deionized or distilled water
• Analytical balance
• Volumetric flask or graduated cylinder
• Stirring bar or glass rod
• Personal protective equipment (gloves, goggles, lab coat)

Procedure

• Step 1
  Decide the target concentration (e.g., 1 M). For a 1 M solution: dissolve **132.14 g** of ammonium sulfate in water and dilute to **1 L** total volume. Adjust the mass proportionally for other final volumes (e.g., 13.214 g for 100 mL of 1 M solution).
• Step 2
  Add the weighed ammonium sulfate solid to a beaker containing ~80 % of the final desired volume of distilled water. Stir until fully dissolved.
• Step 3
  Transfer the solution to a volumetric flask and add water up to the final volume. Mix thoroughly.
• Step 4 (Optional)
  If the solution is intended for sterile applications, filter the solution through a **0.22 µm** membrane into a sterile container.

Note: NH₄⁺ and SO₄²⁻ species contribute to ionic strength; adjust pH only if required by downstream applications. Room temperature storage is generally acceptable for concentrated ammonium sulfate solutions, but clarify any specific requirements for your protocol.

Available Reagents