Preparation Monosodium phosphate Stock Solution
This guide describes the preparation of Monosodium phosphate Stock Solution at a defined molarity for laboratory use.
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Stock Solution Preparation Guide – Monosodium Phosphate (NaH₂PO₄)
Monosodium phosphate (also called Sodium dihydrogen phosphate) can be used to prepare sodium phosphate buffers or other phosphate-based solutions. A 1 M stock solution is frequently used as a component when preparing phosphate buffers of desired pH by mixing with dibasic sodium phosphate.
Chemical Information
| Name | Molecular Formula | Molecular Weight | CAS |
|---|---|---|---|
| Monosodium phosphate, anhydrous | NaH₂PO₄ | 119.98 g/mol | 7558-80-7 |
| Monosodium phosphate monohydrate | NaH₂PO₄·H₂O | 137.99 g/mol | 10049-21-5 |
| Monosodium phosphate dihydrate | NaH₂PO₄·2H₂O | 156.01 g/mol | 13472-35-0 |
Preparation of 1 M Monosodium Phosphate
To prepare 1 L of 1 M sodium phosphate monobasic solution:
- Weigh 119.98 g of anhydrous monosodium phosphate (NaH₂PO₄).
- Transfer to a 2 L beaker or flask.
- Add ~800 mL of deionized or distilled water and stir until fully dissolved.
- Adjust the volume to 1000 mL with deionized water and mix thoroughly.
- Solution should be clear and colorless.
- Optionally, transfer to an autoclavable bottle and autoclave (20 min at 121–124 °C) for sterilization.
Notes and Precautions
- Anhydrous monosodium phosphate is hygroscopic and may absorb water; adjust calculations accordingly.
- Do not dissolve directly into final volume water; additions will increase solution volume.
- Use appropriate personal protective equipment.
Storage and Usage
- Store at room temperature when not in use.
- Use this stock solution directly or mix with sodium phosphate dibasic to prepare buffers of desired pH.
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