Potassium acetate Stock Solution

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Stock Solution Preparation Guide – Potassium Acetate (C₂H₃O₂K) Solution

Potassium acetate is the potassium salt of acetic acid and appears as a white deliquescent crystalline powder that dissolves readily in water. It is widely used as a buffering salt, pH regulator, and in analytical applications. To prepare a 1 M stock solution, dissolve the appropriate mass of potassium acetate in water and adjust to the desired final volume.

Objective

Preparation of a 1 M potassium acetate stock solution.

Composition

• Potassium acetate (C₂H₃O₂K) aqueous solution at defined molarity

Required Materials

• Potassium acetate solid
• Deionized or distilled water
• Analytical balance
• Beaker or flask
• Volumetric flask or graduated cylinder
• Stirring rod or magnetic stirrer
• Personal protective equipment (gloves, goggles, lab coat)

Procedure

• Step 1
  Determine the required mass of potassium acetate. For a 1 M solution: weigh **98.14 g** of potassium acetate (MW = 98.14) to prepare **1 L** of 1 M solution; scale proportionally for other final volumes (e.g., **9.814 g** for 100 mL).
• Step 2
  Place the weighed potassium acetate into a beaker containing about 80 % of the final desired volume of deionized water. Stir until completely dissolved.
• Step 3
  Transfer the dissolved solution into a volumetric flask. Rinse the beaker with small amounts of deionized water to ensure all solute is transferred. Add water to reach the final volume and mix thoroughly.
• Step 4 (Optional)
  If a sterile solution is required for biological use, filter through a **0.22 µm membrane** filter into a sterile container.

Note: Potassium acetate solutions are generally near neutral to slightly alkaline. Label the stock solution with concentration and preparation date; store in a sealed container at room temperature or as required by your protocols.

Available Reagents