Potassium iodide Stock Solution

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Stock Solution Preparation Guide – Potassium Iodide (KI) Solution

Potassium iodide (KI) is a white crystalline salt that dissolves readily in water, dissociating into potassium and iodide ions. It is widely used in analytical chemistry, biological buffers, nutrition studies, and as a reagent in iodometric titrations. To prepare a laboratory stock solution of defined concentration, dissolve measured KI in deionized water and adjust to the desired final volume.

Objective

Preparation of a defined concentration stock solution of potassium iodide (e.g., 1 M).

Composition

• Potassium iodide (KI) aqueous solution at specified molarity

Required Materials

• Potassium iodide (KI) solid
• Deionized or distilled water
• Analytical balance
• Beaker or flask
• Volumetric flask or graduated cylinder
• Stirring rod or magnetic stirrer
• Personal protective equipment (gloves, goggles, lab coat)

Procedure

• Step 1
  Decide the target molarity for your stock solution. For example, to prepare **1 M** KI stock: weigh **166 g** of potassium iodide (MW ≈ 166 g/mol) for **1 L** of solution. Scale proportionally for other volumes (e.g., **16.6 g** for 100 mL of 1 M).
• Step 2
  Add the weighed KI solid to a beaker containing about **80 %** of the target final volume of deionized water. Stir until completely dissolved. Potassium iodide dissolves readily without pH adjustment.
• Step 3
  Transfer the solution to a volumetric flask. Rinse the beaker with deionized water and add the rinses to the flask. Add water up to the final desired volume. Mix thoroughly.
• Optional Sterilization
  If a sterile solution is required (e.g., for cell culture reagents), filter through a **0.22 µm** membrane filter into a sterile container. KI solutions can also withstand autoclaving if necessary.

Note: Potassium iodide is generally light‑stable but over long storage, exposure to air can lead to slight yellowing due to oxidation of iodide to iodine. Store stock solutions in a **tightly sealed container** at room temperature or refrigerated if needed, and clearly label with concentration and date.

Available Reagents