Sodium bicarbonate Stock Solution

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Stock Solution Preparation Guide – Sodium Bicarbonate (NaHCO₃) Solution

Sodium bicarbonate (NaHCO₃), also known as baking soda, is a white crystalline powder that is highly soluble in water and mildly alkaline. A standard 1 M sodium bicarbonate solution has uses in buffering, titration analysis, and pH control. The molecular weight of NaHCO₃ is approximately 84.01 g/mol, and the solution at 25 °C has a pH around 8.3 when at 1 M concentration.

Objective

Preparation of a 1 M sodium bicarbonate stock solution.

Composition

• Sodium bicarbonate (NaHCO₃) aqueous solution of defined molarity

Required Materials

• Sodium bicarbonate solid (NaHCO₃)
• Deionized or distilled water
• Analytical balance
• Beaker or flask
• Volumetric flask or graduated cylinder
• Stirring rod or magnetic stirrer
• Personal protective equipment (gloves, goggles, lab coat)

Procedure

• Step 1
  Decide the target concentration. For a 1 M solution: accurately weigh **84.01 g** of sodium bicarbonate.
• Step 2
  Add the weighed sodium bicarbonate solid to a beaker containing approximately **80 % of the final desired volume** of deionized water (e.g., ~800 mL if preparing 1 L). Stir until completely dissolved.
• Step 3
  Transfer the solution to a volumetric flask and rinse the beaker with small amounts of water, adding rinses to the flask. Add deionized water to reach the final target volume (e.g., 1 L). Mix thoroughly.
• Step 4 (Optional)
  If a sterile solution is required for sensitive applications, filter through an appropriate membrane filter (e.g., 0.22 µm) into a sterile container. Sterilization by heat is generally not necessary for most applications but follow your protocol requirements.

Note: A 1 M NaHCO₃ solution typically has a pH around 8.3 at room temperature. Label the container with concentration and preparation date. Store the stock solution in a sealed container at room temperature or as required.

Available Reagents