Preparation Tris-Glycine Native Running Buffer
This guide describes the preparation of Tris-Glycine Native Running Buffer at a defined molarity for laboratory use.
The following content was generated by AI and has not been strictly verified; it may contain inaccuracies. All information in the BioCalculator App has been manually curated and carefully validated. You can download the App to view it.
Tris-Glycine Native running buffer is commonly used for native polyacrylamide gel electrophoresis, allowing protein separation while preserving native conformation and biological activity.
Tris-Glycine Native Running Buffer – 1X Working Solution Composition
| Name | Formula | Concentration (1X) | CAS |
|---|---|---|---|
| Tris base | C₄H₁₁NO₃ | 25 mM | 77-86-1 |
| Glycine | NH₂CH₂COOH | 192 mM | 56-40-6 |
Applications of Tris-Glycine Native Running Buffer
Tris-Glycine Native running buffer is used for non-denaturing (native) PAGE, enabling the separation of proteins or protein complexes based on their charge, size, and conformation while maintaining native structure and biological activity.
Preparation Tips, Sterilization, and Storage
- The pH of the solution should be 8.3 and does not require any adjustment.
- Sterilization: usually not required; choose as needed.
- Storage: room temperature.
