Preparation ATP Stock Solution
This guide describes the preparation of ATP Stock Solution at a defined molarity for laboratory use.
The following content was generated by AI and has not been strictly verified; it may contain inaccuracies. All information in the BioCalculator App has been manually curated and carefully validated. You can download the App to view it.
Stock Solution Preparation Guide – ATP (Disodium Salt)
ATP disodium salt (Adenosine 5′-triphosphate disodium salt) is the biologically active form of ATP commonly used as an energy substrate in enzymatic assays, cell metabolism studies, and molecular biology applications. It participates in phosphorylation reactions and serves as the universal energy currency in cells.
Chemical Information
- CAS Number: 987-65-5
- Molecular Formula: C10H14N5O13P3Na2
- Molecular Weight: 551.14 g/mol
- Name: ATP disodium salt
Solubility and Handling
ATP disodium salt is readily soluble in water (e.g., ≥ 50 mg/mL), forming a clear solution suitable for buffer and enzymatic assay preparation.
- Prepare ATP stock solution in purified water; warming and gentle mixing can help dissolution.
- ATP solutions are sensitive to hydrolysis, especially at elevated temperatures or extreme pH. Avoid prolonged storage at room temperature.
Stock Solution Preparation
Below is an example for preparing a 100 mM ATP stock solution:
• Weigh out 55.115 g of ATP disodium salt.
• Add to ~800 mL of deionized water and dissolve completely.
• Adjust pH if required (e.g., pH 7.0–7.5) using NaOH or HCl.
• Bring to final volume of 1 L with deionized water.
Once dissolved, aliquot and store ATP stock at −20°C or lower to minimize degradation and avoid repeated freeze–thaw cycles.
Storage and Usage
- Store solid ATP disodium salt at −20°C with desiccant.
- Dissolved ATP stock solutions can be kept at −20°C for weeks to months; avoid frequent freeze–thaw.
- Use sterile technique for cell culture or enzymatic applications.
